The 3′ enhancer region determines the B/T specificity and pro-B/pre-B specificity of immunoglobulin Vκ-Jκ joining

Ryuji Hiramatsu,’ Kiwamu Akagi,t Masao Matsuoka,* Kunihiko Sakumi,’ Hiro Nakamura,* Linda Kingsbury,’ Chella David,* Richard R. Hardy,5 Ken-ichi Yamamura,t and Hitoshi Sakano’ll *Division of Immunology Department of Molecular and Cell Biology University of California, Berkeley Berkeley, California 94720-3200 tDepartment of Developmental Genetics Institute of Molecular Embryology and Genetics Kumamoto University School of Medicine Kumamoto 862 Japan *Department of Immunology Mayo Clinic Rochester, Minnesota 55905 llnstitute for Cancer Research Fox Chase Cancer Center Philadelphia, Pennsylvania 19111 IlDepartment of Biophysics and Biochemistry Graduate School of Science The University of Tokyo Bunkyo-ku Tokyo 113 Japan